Couldn’t make it to AGBT (again), so I’m unfortunately limited to monitoring FF Twitter and my RSS feed for coverage. Figured I might as well share my finds.
Pre-meeting
General notes
- New Solid sequencer
- Semiconductor sequencing about to be released
- New Illumina chemistry: 150 bp reads and new chemistry —> complete 30X human genome (95 GB) in single run.
Talk notes
- Richard Gibbs estimates 1,000 personal genomes will be produced in 2010, but not yet reaching high quality. Data production and computation flip flop; datastream comes in lurches, as opposed to graduate growth of computation. Computational resources being outpaced by data accumulation; we need to learn how to throw away data.
- Debbie Nickerson, University of Washington, discussing experiences of data production at a sequencing facility. We’re likely to see 10,000 exomes in 2010 in addition to the 1,000 complete genomes predicted by Richard Gibbs. array-based sequence capture techniques are impossible to scale; solution-based capture more reproducible, efficient (less variation in quality).
- Kelly Frazer just gave a very interesting talk on choosing the right sequence capture method. Short answer: it depends. Sorry…
- Wold recommends computation for chip/rna-seq article
